We organize courses for bioinformaticians interested in analyzing their own data, both internally and abroad within the European network ELIXIR.Īll data and metadata are collected and managed in comprehensive sample and library tracking database that allows us to adhere to the FAIR principles. We train researchers on the required experimental procedures and equipment. Data management, including backup, live progress overviews, quality reports and downstream analyses.Expertise, including advice on experimental design, planning and trouble shooting.After an intake meeting where goals, feasibility and mutual expectations are discussed, we can provide: The Single Cell Genomics facility makes single cell technologies available to researchers of the Princess Máxima Center. Visium: spatial transcriptomics, measuring the local gene expression in microscopic images.V(D)J sequencing: simultaneous gene expression and T- and B-cell receptor sequencing.Cellplex: multiplexed gene expression, in which samples are pooled for higher throughput and reduced cost.Feature Barcoding: simultaneous gene expression and cell surface protein quantification.Multiome: simultaneous gene expression and chromatin-accessibility per cell.Lastly, the 10x Genomics platform allows a variety of single-cell quantification strategies: Mission Bio Tapestri offers single-cell DNA sequencing, allowing the identification of SNVs, indels, CNVs and other structural variants in individual cells. SORTseq is a technique for performing gene expression profiling of single cells that are FACS-sorted into 384-well plates. We facilitate both single-cell and single-nucleus RNA and DNA sequencing using three platforms.
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